Spatially-controlled ligand aarrangement by origami-based nanoprinters (EU-H2020 ERC-2020-StG-950421)

SPACING aims to develop a state-of-the-art technology for precise ligand assembly onto nanoparticles, inspired by natural structures like virus capsids. Utilizing DNA origami-based nanoprinters, the project will fabricate libraries of NPs and pre-designed ligand configurations. Through proof-of-concept experiments, SPACING will assess the trafficking behavior of these bio-inspired NPs within cells, focusing on their potential to evade lysosomal degradation. Ultimately, the project seeks to establish the foundation for artificial NPs with tailored 3D ligand configurations, with implications for advanced drug delivery and biomedical applications.